Determining the analytical sensitivity of polymerase chain reaction targeting Ehrlichia spp. disulfide oxidoreductase gene: Molecular Diagnosis of ehrlichiosis in a dog clinically suspected with leishmaniasis

Ehrlichia spp. is tick-borne zoonotic pathogen that can infect humans and animals. Nowadays, among the tests used in diagnosis of ehrlichiosis, importance molecular methods increasing steadily due to their high sensitivity specificity. The aim this study was determine analytical a conventional polymerase chain reaction (PCR) targeting disulfide oxidoreductase (DSB) gene. DSB gene cloned into TOPO vector. After plasmid containing were serially diluted, PCR performed. While working on research, blood skin scraping samples stray dog clinically suspected with leishmaniasis as well treated for arrived our laboratory. Thereafter, PCRs 16S rRNA Leishmania kinetoplast DNA (kDNA) genes performed identify dog. assay 1 ≥ copy plasmid/reaction using diluted positive kDNA negative leishmaniasis. Using nested rRNA, E. canis identified In study, has been shown have sensitivity. Also it help clinicians differential ehrlichiosis prevent inappropriate treatment.